Journal: Frontiers in Microbiology

Article Title: Licosin, a multifunctional defensin peptide originated from the clinical fungus Lichtheimia corymbifera with antibacterial and potassium ion channel blocking effects

doi: 10.3389/fmicb.2026.1808106

Figure Lengend Snippet: Gene cloning, sequence, and evolution analysis of licosin. (A) Amplification of the licosin gene and coding sequence from clinical pathogenic fungi L. corymbifera . Lane M: DNA marker; Lane 1: Amplification products using fungal genomic DNA as a template; Lane 2: Amplification products using first-strand cDNA as a template. (B) The CDS and the corresponding amino acid sequence of licosin. The putative signal peptide (positions 1–20) and precursor peptide (positions 21–27) are indicated by red and blue rectangle boxes, respectively. (C) Sequence alignment of licosin and other peptides from fungi, plants, insects, and primates. The cysteines and basic residues are shaded in brilliant yellow and blue, respectively. The position between the precursor peptide (positions 21–27) and the mature peptide (positions 28–68) is indicated by a red arrow. (D) Phylogenetic tree based on the sequence alignment of licosin and other defensin peptides. The percentage of bootstrap support was calculated and displayed on the branch lines.

Article Snippet: The fungal genomic DNA was extracted using the Fungi/Yeast Genomic DNA Isolation Kit (Sangon Biotech, Shanghai) following the manufacturer’s instructions.

Techniques: Cloning, Sequencing, Amplification, Marker